Ines Lopez-Calleja Diaz
Post Doc

C U R R E N T  R E S E A R C H / I N T E R E S T S

PCR and real time PCR to identify animal species in milk mixtures and cheeses Department of Food Science and Technology

E D U C A T I O N

• PhD in Veterinary Medicine “Cum Laude” 2001-2007 European PhD School of Veterinary Medicine at U.C.M. (Universidad Complutense de Madrid

• Bachelor of Veterinary Medicine 1996-2001 “Animal Health and Medicine” speciality School of Veterinary Medicine at U.C.M

P U B L I C A T I O N S

1. López-Calleja, I., González, I., Fajardo, V., Martín, I., Hernández, P.E., García, T. & Martín, R. (2007) “Quantitative detection of goats’ milk in sheep’s milk by real-time PCR” Food Control 18, 1466-1473.
2. López-Calleja, I., González, I., Fajardo, V., Martín, I., Hernández, P.E., García, T. & Martín, R. (2007) “Real-time taqman PCR for quantitative detection of cows’ milk in ewes’ milk mixtures” International Dairy Journal 17, 729-736.
3. López-Calleja, I., González, I., Fajardo, V., Martín, I., Hernández, P.E., García, T. & Martín, R. (2007) “Application of a Polymerase Chain Reaction to detect adulteration of ovine cheeses with caprine milk” European Food Research and Technology 225, 345-349.
4. López-Calleja, I., González, I., Fajardo, V., Hernández, P.E., García, T. & Martín, R. (2007) “Application of an indirect ELISA and a PCR technique for detection of cows’ milk in sheep’s and goats’ milk cheeses” International Dairy Journal 17, 87-93.
5. Martín, I., García, T., Fajardo, V., López-Calleja, I., Rojas, M., Pavón, M.A., Hernández, P.E., González, I. & Martín, R. (2007) “Mitochondrial markers for the detection of four duck species and the specific identification of Muscovy duck in meat mixtures using the polymerase chain reaction” Meat Science 76, 721-729.
6. Fajardo, V., González, I., López-Calleja, I., Martín, I., Rojas, M., Hernández, P.E., García, T. & Martín, R. (2007) “Real-time PCR for detection and quantification of red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) in meat mixtures” Meat Science (sent).
7. Fajardo, V., González, I., López-Calleja, I., Martín, I., Rojas, M., Hernández, P.E., García, T. & Martín, R. (2007) “Differentiation of European wild boar (Sus scrofa scrofa) and domestic swine (Sus scrofa domestica) meats by PCR analysis targeting the mitochondrial D-loop and the nuclear melanocortin receptor 1 (MC1R) genes” Meat Science (in press).
8. Fajardo, V., González, I., López-Calleja, I., Martín, I., Rojas, M., Hernández, P.E., García, T. & Martín, R. (2007) “PCR identification of meats from chamois (rupicapra rupicapra), pyrenean ibex (capra pyrenaica), and mouflon (ovis ammon) targeting specific sequences from the mitochondrial d-loop region” Meat Science 76, 644-652.
9. Fajardo, V., González, I., López-Calleja, I., Martín, I., Rojas, M., Pavón, M., Hernández, P.E., García, T. & Martín, R. (2007) “Analysis of Mitochondrial DNA for Authentication of Meats from Chamois (Rupicapra rupicapra), Pyrenean Ibex (Capra pyrenaica), and Mouflon (Ovis ammon) by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism” Journal of AOAC International 90, 179-186.
10. Fajardo, V., González, I., López-Calleja, I., Martín, I., Hernández, P.E., García, T. & Martín, R. (2007) “Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama) and roe deer (Capreolus capreolus) using Polymerase Chain Reaction (PCR) targeting specific sequences from the mithocondrial 12S rRNA gene” Meat Science 76, 234-240.
11. Martín, I., García, T., Fajardo, V., López-Calleja, I., Hernández, P.E., González, I. & Martín, R. (2007) “Species-specific PCR for the identification of ruminant species in feedstuffs” Meat Science 75, 120-127.
12. Martín, I., García, T., Fajardo, V., López-Calleja, I., Rojas, M. & Pavón, M.A., Hernández, P.E., González, I. & Martín, R. (2007) “Technical note: Detection of chicken, turkey, duck and goose tissues in feedstuffs using species-specific PCR” Journal of Animal Science 85, 452-458.
13. Fajardo, V., González, I., López-Calleja, I., Martín, I., Hernández, P.E., García, T. & Martín, R. (2006) “PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries) and goat (Capra hircus)” Journal of Agricultural and Food Chemistry 54, 1144-1150.
14. López-Calleja, I., González, I., Fajardo, V., Martín, I., Hernández, P.E., García, T. & Martín, R. (2005) “Application of Polymerase Chain Reaction to detect adulteration of sheep’s milk with goats’ milk” Journal of Dairy Science 88, 3115-3120.
15. López-Calleja, I., González, I., Fajardo, V., Rodríguez, M.A., Hernández, P.E., García, T. & Martín, R. (2005) “PCR detection of cows’ milk in water buffalo milk and mozzarella cheese” International Dairy Journal 15, 1122-1129.
16. López-Calleja, I., González, I., Fajardo, V., Rodríguez, M.A., Hernández, P.E., García, T. & Martín, R. (2004) “Rapid detection of cows’ milk in sheep’s and goats’ milk by a species-specific PCR technique” Journal of Dairy Science 87, 2839-2845.
17. García, T., Mayoral, M.B., González, I., López-Calleja, I., Sanz, A., Hernández, P.E. & Martín, R. (2004) “Enumeration of yeasts in dairy products: A comparison of immunological and genetic techniques” Journal of Food Protection 67, 357-364.
18. Rodríguez, M. A., García, T., González, I., Asensio, L., Mayoral, B., López-Calleja, I., Hernández, P. E. & Martín, R. (2003) “Development of a Polymerase Chain Reaction assay for species identification of goose and mule duck in foie gras products” Meat Science 65, 1257-1263.
19. Rodríguez, M. A., García, T., González, I., Asensio, L., Mayoral, B., López-Calleja, I., Hernández, P. E. & Martín, R. (2003) “Identification of goose, mule duck, chicken, turkey and swine in foie gras by species-specific Polymerase Chain Reaction” Journal of Agricultural and Food Chemistry 51, 1524-1529.
20. Asensio, L., González, I., Rodríguez, M. A., Mayoral, B., López-Calleja, I., Hernández, P. E., García, T. & Martín, R. (2003) “Development of a specific monoclonal antibody for grouper (Epinephelus guaza) identification using an Indirect Enzyme-Linked Immunosorbent Assay” Journal of Food Protection 66, 886-869.
21. Asensio, L., González, I., Rodríguez, M. A., Mayoral, B., López-Calleja, I., Hernández, P. E., García, T. & Martín, R. (2003) “Identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus) and Nile perch (Lates niloticus) fillets by polyclonal antibody-based ELISA” Journal of Agricultural and Food Chemistry 51, 1169-1172.

C O N T A C T  I N F O

Cornell University/Ludwig Institute for Cancer Research Partnership
318 Stocking Hall
Cornell University
Ithaca, NY 14853
Tel: (607) 255-7902
FAX: (607) 255-8741

email: ineslopezcalleja@gmail.com