F R A N K   K.   N.   L E E
ASSISTANT INVESTIGATOR

C U R R E N T  R E S E A R C H / I N T E R E S T S

My current responsibilities include coordinating the validation, operation and compliance of the Biologics Production Facility (BPF). The CU/LICR Partnership BPF is a cGMP compliant facility dedicated to the production of recombinant protein immunotherapeutics for use in early human clinical trials.

My previous research activities in the Ludwig laboratory included the expression and purification of a humanized Fab molecule from E. coli and Pichia pastoris, and expression and purification of an scFv (single-chain antibody) molecule from E. coli.

E D U C A T I O N

University of Iowa, Iowa City, IA, USA
[Completed 1998]
Ph.D., Microbiology

McMaster University, Hamilton, ON, CANADA
[Completed 1989]
M.S., Biology

University of Guelph, Guelph, ON, CANADA
[Completed 1985]
B.Sc. (Honors)

P O S T D O C T O R A L   A P P O I N T M E N T S

Department of Microbiology, University of Iowa, Iowa City, IA
Postdoctoral Research Associate

H O N O R S  &   A W A R D S

American Society for Microbiology Sustaining Member Student Travel Grant (1997)

C E R T I F I C A T I O N S

Complying with GMPs for Clinical Manufacturing. Pharmaceutical Training Institute. (05/2002)

Good Validation Practice for Biologicals, Biopharmaceutical and Synthetic Drugs. IBC USA Conferences, Inc. (06/2002)

P U B L I C A T I O N S

Zaleski, A., K. Scheffler, P. Densen, F. K. N. Lee, M. Ketterer, B. W. Gibson, and M. A. Apicella. The lipooligosaccharide Pk (Gal?1-4Gal?1-4Glc) epitope of Moraxella catarrhalis is a factor in the resistance to the bactericidal activity mediated by normal human serum. Accepted for publication in Infection and Immunity.

Lee, F. K. N., B. W. Gibson, W. Melaugh, M. A. Apicella. 1999. Relationship between UDP-glucose 4-epimerase activity and oligoglucose glycoforms in two strains of Neisseria meningitidis. Infect. Immun. 67(3):1405-14

Lee, F. K. N., K.C. Dudas, J.A. Hanson, M.B. Nelson, P.T. LoVerde and M.A. Apicella. 1999. The R-pyocin of Pseudomonas aeruginosa strain C is a bacteriophage tail-like particle which contains single-stranded DNA. Infect. Immun. 67(2):717-25

Apicella, M. A., M. Ketterer, F.K. Lee, D. Zhou, P.A. Rice and M.S. Blake. 1996. The pathogenesis of gonococcal urethritis in men: confocal and immunoelectron microscopic analysis of urethral exudates from men infected with Neisseria gonorrhoeae. J. Infect. Dis. 173(3):636-46.

Lee, F.K.N., D.S. Stephens, B.W. Gibson, J.J. Engstrom, D. Zhou, and M.A. Apicella 1995 The microheterogeneity of Neisseria lipooligosaccharide: Analysis of a UDP-Glucose 4-epimerase mutant of Neisseria meningitidis strain NMB. Infect. Immun. 63(7):2508-15.

Stephens, D.S., C. F. McAllister, D. Zhou, F. K. Lee, and M.A. Apicella. 1994 Tn916 generated lipooligosaccharide mutants of Neisseria meningitidis. Infect. Immun. 62:2947-2952

Zhou, D., D. S. Stephens, B. W. Gibson, J. J. Engstrom, C. F. McAllister, F. K. Lee, and M. A. Apicella. 1994. Lipooligosaccharide biosynthesis in pathogenic Neisseria. Cloning, identification, and characterization of the phosphoglucomutase gene. J. Biol. Chem. 269:11162-9

A B S T R A C T S  &  P R E S E N T A T I O N S

Lee, F. K. N., M. D. Wolfe, J. D. Lipscomb, and D. T. Gibson. 2000 Biochemical characterization of the reductase component of 2-nitrotoluene dioxygenase. Annual meeting of Society for Industrial Microbiology.

Lee, F. K. N., J. V. Parales and D. T. Gibson. 2000 The purification and characterization of 2-nitrotoluene 2,3-dioxygenase reductase from Pseudomonas sp. JS42. Abstracts of the 100th General Meeting of the American Society for Microbiology.

Lee, F. K. N. and M. A. Apicella. 1997 Molecular basis for differential UDP-galactose 4-epimerase activity levels in three serogroups of Neisseria meningitidis. Abstracts of the 97th General Meeting of the American Society for Microbiology.

Lee, F. K. N., K. C. Dudas, J. A. Hanson, M. B. Nelson, P. T. LoVerde and M. A. Apicella. 1995. The R-Pyocin of Pseudomonas aeruginosa strain C is a Bacteriophage Tail-Like Particle Which Contains Single-Stranded DNA. Third Annual Midwest Bacterial Pathogenesis Conference. Madison, WI.

Zhou, D., F. K. Lee, H. Harvey and M. A. Apicella 1995 A thirty-two bp A+T-rich region in the putative promoter region and a sixteen bp palindrome sequence encompassing the putative ribosomal binding site are conserved in pathogenic Neisseria rfaC genes. Abstracts of the 95th General Meeting of the American Society for Microbiology.

Lee, F.K. and M.A. Apicella. 1994 Isolation of a S. typhimurium rfaE homologue in Neisseria gonorrhoeae. Proceedings of the Ninth International Pathogenic Neisseria Conference. Winchester, England

Lee, F.K., D. Zhou, M. Ketterer, M.A. Apicella. 1994 Increased expression of lipooligosaccharide during gonococcal urethral infection in males. Abstracts of the 94th General Meeting of the American Society for Microbiology.

Lee, F.K., M.A. Apicella, C.F. McAllister, D.S. Stephens. 1993 Tn916 insertion in a galE homologue allows identification of lipooligosaccharide biosynthesis regions in pathogenic Neisseria. Abstracts of the 93rd General Meeting of the American Society for Microbiology.

Lee, F.K.N., K.C. Dudas, and M.A. Apicella. 1992 Integration of pyocin DNA into the genome of Neisseria gonorrhoeae. Pathobiology and Immunobiology of Neisseriaceae. Proceedings of the Eighth International Pathogenic Neisseria Conference. Cuernavaca, Mexico.

Lambert, G., M. Hamel, R. Carriero, E. Deroukakis, F. Lee, M. Wosnick and M. Dennis. 1990. Expression of the Human 5-HT1a receptor in insect cells using a baculovirus receptor. The Second IUPHAR Satellite Meeting on Serotonin. Basel Switzerland.

MEMBERSHIPS, AFFILIATIONS, ASSOCIATIONS

American Society for Microbiology (ASM) Member

Society of Industrial Microbiology (SIM) Member

C O N T A C T  I N F O

Cornell University/Ludwig Institute for Cancer Research Partnership
307 Stocking Hall
Cornell University
Ithaca, NY 14853
Tel: (607) 254-6382
FAX: (607) 255-8741

email: FKL3@cornell.edu